Aishwarya Gangadhar
I'm a Biotechnologist
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I am a graduate researcher specialized in medical biotechnology with extensive experience in nanomedicine, immunology, and molecular biology, aiming to make a difference in the field of research and contribute to improving the quality of life for mankind.
Master of Science in Medical Biotechnology
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August 2022-May 2024
Bachelor of Technology in Biotechnology (Honors)
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August 2018-May 2022
Aseptic techniques, Mammalian Cell culture, Cryopreservation, Cancer biology/immunology assays: colony formation, spheroid assay, wound healing assay, immunocytochemistry, Trans-well, migration assay, cytotoxicity assays.
Polymeric, Lipid nanoparticle (LNP) synthesis, Characterization (Dynamic Light Scattering (DLS), SEM). Evaluating encapsulation efficiency, Release Kinetics.
Protein quantification assays, Protein purification & modification, Protein desalting Western blotting.
DNA, RNA isolation, PCR, cDNA synthesis, Agarose gel electrophoresis, Real-time PCR, Cloning, transformation, & restriction digestion.
ELISA, Immunocytochemistry, Cytokine Bead Array (CBA) assay, Flow cytometry (BD FACS Calibur) & Magnetic-activated Cell Sorting (MACS).
Zebrafish husbandry, Mating event set-up, Toxicity evaluation through zebrafish embryos, Live-Imaging of embryos, Execution of zebrafish embryo experiments.
Mice restraining, Optical imaging (UVP IBox), Tail snipping, Blood sample collection, and Injection techniques (IP, IV, IM, SC, RO).
Fluorescence microscopy, Confocal microscopy, Scanning electron microscopy, Spectrophotometry, Dynamic Light Scattering (DLS).
GraphPad Prism, OriginPro, ImageJ, Minitab, Microsoft Office (Word, Excel & PowerPoint), Biorender.
- Established Diabetic Retinopathy (DR) disease model in Zebrafish embryos.
- DR model validated by H & E staining, hyaloid retinal vasculature imaging, cardiac rhythm measurement, RNA isolation, qPCR, and toxicity study.
- Synthesized and characterized (zeta potential, DLS) polymeric nanoparticle (PEG-b-PPS) micelles.
- Assessed the uptake of tracking dye-loaded nanoparticles.
- Evaluated the biocompatibility of micelles in DR model zebrafish embryos through flow cytometry analysis, AO staining, and toxicity study.
- Assessed the anti-angiogenesis efficiency of Micelles in DR model embryos.
Skills: qPCR, RNA isolation, gel electrophoresis, H and E staining, toxicity studies, AO staining, Cell cycle analysisFlow cytometry.
Immunofluorescence, microscopy, nanoparticle synthesis, zeta potential measurement, and characterization (DLS, SEM).
- Evaluated alterations in the expression levels of predicted binding targets of tryptamine in neuronal cells following treatment
with gold-coated tryptamine liposomes and free tryptamine, employing immunocytochemistry techniques.
Skills: LNP synthesis, characterization, evaluating encapsulation efficiency, release kinetics, immunofluorescence.
- Executed adoptive transfer of splenocytes from ApoE Foxp3 to Rag -/- mice, monitoring immune cell reconstitution over 12 weeks.
- Conducted weekly tail snip bleeding and analyzed PBMC, emphasizing diverse B and T lymphocytes via multi-color flow cytometry.
- Organizing lab sessions and training 20 students in various biochemical techniques such as
protein assays, protein purification, modification, chromatography, electrophoresis, and conjugation chemistry
Conducted and organized laboratory sessions and provided guidance and training to students in fundamental laboratory skills such as
pipetting, protein assays, mammalian cell culture, cryopreservation, as well as cell assays.
Oversee the maintenance of multiple university laboratories, ensuring the calibration and basic repair of diverse laboratory equipment.
Collaborate closely with professors to comprehend practical class needs, prepare necessary materials.
Efficiently manage inventory, including procurement from vendors, and undertake cleanup responsibilities post-classes.
Manage and oversee the daily operations of the department cafeteria, ensuring a well-maintained environment.
Received training and actively conducted microbiological tests for the validation of sterile pharmaceuticals.
Evaluating Biocompatibility and Anti-Angiogenesis Efficiency of Anti-Integrin PEG-b-PPS Micelles in Danio rerio Diabetic Retinopathy Model.” at 31st International Precision Medicine TRI-CON Conference, March 26-28, 2024, San Diego, CA, USA.
In vivo anti-angiogenic therapeutic efficiency of SDV peptide decorated PEG-b-PPS Micelles in Danio rerio Diabetic Retinopathy Model”. at UIC Health Sciences Rockford’s 29th Annual Research Day, April 11, 12 2024, UIC College of Medicine Rockford, Rockford, IL, USA.
In vivo anti-angiogenic therapeutic efficiency of SDV peptide decorated PEG-b-PPS Micelles in Danio rerio Diabetic Retinopathy Model”. at 10th IBTN Research Symposium, April 29th 2024, UIC College of Dentistry, Chicago, IL.
Evaluating Biocompatibility and Anti-Angiogenesis Efficiency of Anti-Integrin PEG-b-PPS Micelles in Danio rerio Diabetic Retinopathy model.” at 5th International Conference on Materials Science & Engineering, May 10-13 2024, San Francisco, CA.
Best Basic Science Research Poster Award at the 29th Annual Research Day at UIC College of Medicine Rockford.
1st place for Best Poster Presentation at the 10th IBTN Research Symposium at UIC College of Dentistry.
